![]() In most PAGE applications, the gel is mounted between two buffer chambers, and the only electrical path between the two buffers is through the gel. This difference in migration rate between smaller and larger proteins leads to their physical separation in the gel. As proteins move through the gel in response to an electric field, the gel’s pore structure allows smaller proteins to travel more rapidly than larger proteins. This polyacrylamide gel is what lends the name to the technique: polyacrylamide gel electrophoresis, or PAGE. Polyacrylamide Gel Electrophoresis (PAGE) OverviewĪlmost all methods for protein separation use an aqueous polyacrylamide gel as a size-selective sieve during separation.
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